Phospho-EphA2 (Tyr594) Antibody
货号：
3970S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3970S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-EphA2 (Tyr594) Antibody detects transfected levels of EphA2 proteins only when phosphorylated on Tyr594. This antibody does not cross-reacted with other activated protein tyrosine kinases. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, the ephrins stimulate the kinase activity of the Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyrosines 324/327 have been identified as major phosphorylation sites of ephrin B1 in vivo (7). |
| 存放说明 | -20C |
| 计算分子量 | 135 |
参考图片Western blot analysis of cell extracts from 293-T cells, transfected with human EphA2 expression construct, untreated or stimulated with B61-Fc ligand, using Phospho-EphA2 (Tyr594) Antibody (upper) or EphA2 Antibody #3974 (lower).用抗磷酸化EphA2 (Tyr594)的抗体（上）或抗EphA2的抗体 #3974（下）对经过或未经B61-Fc配体刺激的转染了人EphA2表达组件的293-T细胞的裂解液进行免疫印迹检测。 |
