| 货号 | 9574S |
| 反应种属 | Human/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-eNOS (Thr495) Antibody detects endogenous levels of eNOS only when phosphorylated at threonine 495. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Western blot analysis of extracts from porcine aortic endothelial cells (PAECs), 3 days post-confluence, untreated, H2O2-treated (0.3 mM for 30 minutes) or calf intestinal alkaline phosphatase (CIP)-treated as indicated, using Phospho-eNOS (Thr495) Antibody (upper) or control eNOS antibody (lower). Western blot分析猪动脉内皮细胞（PAECs） ，汇合后3天，未处理组和H2O处理组(0.3 mM，30 分钟)或小牛肠碱性磷酸酶处理组，所用抗体为Phospho-eNOS (Thr495) Antibody (上)或对照 eNOS antibody (下)。 |
