Phospho-eIF4E (Ser209) Antibody
货号：
9741S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 9741S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-eIF4E (Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at serine 209. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. |
| 存放说明 | -20C |
| 计算分子量 | 25 |
参考图片Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum, PD98059 or Dexamethasone, using Phospho-eIF4E (Ser209) Antibody (upper) or eIF4E Antibody #9742 (lower). 使用Phospho-eIF4E (Ser209) Antibody (上图)和eIF4E Antibody #9742 (下图)，免疫印迹（Western Blot）分析在NIH/3T3细胞系中Phospho-eIF4E (Ser209)和eIF4E蛋白水平，细胞分为对照组，PD98059处理组和Dexamethasone处理组。 |
