Phospho-eIF2α (Ser51) Antibody
货号：
9721S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 9721S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 38 |
参考图片Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM), using Phospho-eIF2alpha (Ser51) Antibody (upper) or eIF2alpha Antibody #9722 (lower). 使用Phospho-eIF2alpha (Ser51) Antibody (上图)和eIF2alpha Antibody #9722 (下图)，免疫印迹（Western Blot）分析在PC12细胞系中Phospho-eIF2α (Ser51)和eIF2alpha蛋白水平水平，细胞分为对照组和thapsigargin(300 nM)处理组。 |
