Phospho-EGF Receptor (Ser1046/1047) Antibody
货号：
2238S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2238S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
参考图片Western blot analysis of extracts from A431 cells, untreated or stimulated with EGF (100 ng/ml, 10 min), using Phospho-EGF Receptor (Ser1046/1047) Antibody (upper) or EGF Antibody #2232 (lower). Calf intestinal phosphatase (CIP) treatment abolished the immunoreactivity of Phospho-EGF Receptor (Ser1046/1047) Antibody with EGF-stimulated A431 cell lysates (middle).用下列抗体对经过或未经EGF (100 ng/ml, 10 min) 处理的A431细胞的裂解液进行免疫印迹检测： 抗磷酸化EGF受体(Ser1046/1047) 的抗体（上）或抗EGF受体的抗体#2232 (下) 。牛小肠磷酸酶(CIP) 预处理能够破坏抗磷酸化EGF受体(Ser1046/1047) 的抗体与EGF刺激过的A431细胞的裂解液发生免疫反应（中）。 |
