| 货号 | 4867S |
| 反应种属 | Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-DRP1 (Ser637) Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser637. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Dynamin-related protein 1 (DRP1) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DRP1 affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DRP1 clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis as well as normal cell growth and development, is controlled, in part, by the phosphorylation of DRP1 at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DRP1 stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DRP1 is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation of Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DRP1 is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Mitochondrial defects are seen in a variety of neurodegenerative diseases including Alzheimers disease, Parkinsons disease, and Huntingtons disease (reviewed in 6). |
| 存放说明 | -20C |
| 计算分子量 | 78-82 |
Western blot analysis of extracts from PC-12 cells, untreated or treated with 20uM Forskolin #3828 for 1 hour, using Phospho-DRP1 (Ser637) Antibody.使用Phospho-DRP1 (Ser637) Antibody，免疫印迹（Western Blot）分析PC-12细胞中Phospho-DRP1 (Ser637)蛋白水平。细胞分为未处理组和20uM Forskolin #3828处理1 h组。 | |
Immunoprecipitation of PC-12 cell lysates, untreated or treated with 20 μM Forskolin #3828 for 1 hour, using Phospho-DRP1 (Ser637) Antibody. The western blot was probed using the same antibody. Lanes 1 and 2 are 5% input.使用Phospho-DRP1 (Ser637) Antibody，免疫沉淀（Immunoprecipitation）分析PC-12细胞裂解物，细胞分为未处理组和20uM Forskolin #3828处理1 h组。使用同样的抗体进行免疫印迹（Western blot）实验。第1和2道是5% input。 |
