Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody
货号：
9209S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 9209S |
| 反应种属 | Human/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody detects endogenous levels of Drosophila p70 S6 kinase only when phosphorylated at threonine 398. The antibody will also recognize human p70 S6 Kinase when phosphorylated at threonine 389. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5 oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal (1). Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade (1). The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (1). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (1). Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo (3). Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 (4,5). Phosphorylation of this site is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI-3K inhibitor) and rapamycin (FRAP/mTOR inhibitor) (1,6,7). Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region (1). Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression (1,2). Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase (8).Drosophila p70 S6 kinase (dS6K) is a critical regulator of cell growth, but this effect is independent of Akt and PI-3K signaling (9,10). However, insulin-induced activation and phosphorylation of dS6K at Thr398 is dependent on PI-3K, mTOR, and Akt (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
参考图片Western blot analysis of extracts from Drosophila S2 cells and Human MCF-7 cells, using Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody (upper), Phospho-p70 S6 Kinase (Thr389) Antibody #9205, which recognizes the human, mouse and rat protein (middle), or Akt Antibody #9272 (lower). Serum-starved S2 cells were untreated or treated with EGF (100 ng/ml, 30min) #9908. Serum-starved MCF-7 cells were untreated or treated with IGF-1 (50 ng/ml) in the presence or absence of LY294002 (10 uM) #9901. Western blot分析果蝇S2细胞和人MCF-7细胞提取物，所用抗体为Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody (上), Phospho-p70 S6 Kinase (Thr389) Antibody #9205, 可以识别人类、小鼠和大鼠蛋白 (中) 或 Akt Antibody #9272 (下)。血清饥饿的S2细胞分为未处理组和用EGF (100 ng/ml, 30min) #9908处理组。血清饥饿的MCF-7细胞在LY294002 (10 uM) #9901存在和缺失的条件下，分为未处理组和采用IGF-1 (50 ng/ml)处理组。 |
