Phospho-Drosophila Akt (Ser505) Antibody
货号：
4054S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4054S |
| 反应种属 | D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho Drosophila Akt (Ser505) Antibody detects endogenous levels of Akt only when phosphorylated at serine 505. It does not recognize drosophila Akt when phosphorylated at other sites, nor does it recognize related kinases such as PKC or p70 S6 Kinase. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).Drosophila Akt (D-Akt) regulates multiple biological processes such as cell survival (20) and cell growth (21). Major sites of phosphorylation include Ser505 and Thr342. These activation sites are homologous to mammalian Ser473 and Thr308 respectively. Identified downstream targets of D-Akt include Trh (Ser665) (22) and Tsc2 (23). Like Akt, D-Akt functions in a wortmannin-sensitive pathway involving PI3 kinase (24). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
参考图片Western blot analysis of extracts from serum-starved (6 hours) S2 cells treated with 10 ug/ml insulin with or without 1 uM wortmannin pretreatment, using Phospho-Drosophila Akt (Ser505) Antibody (top) or Akt antibody #9272 (bottom). Western blot 分析10 ug/ml胰岛素处理的血清饥饿（6小时）S2细胞提取物，并分别为应用渥曼青霉预处理或者未预处理组，使用的抗体为Phospho-Drosophila Akt (Ser505) Antibody(上图) 或者 Akt antibody #9272 (下图)。 |
