| 货号 | 3453S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | Phospho-Doublecortin (Ser334) Antibody detects endogenous levels of doublecortin only when phosphorylated at Ser334. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:200) |
| 供应商 | CST |
| 背景 | Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).Doublecortin phosphorylated at Ser334 is enriched in growth cones and affects neurite outgrowth and neuronal migration (3). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Phospho-Doublecortin (Ser334) Antibody. The phospho-specificity of the antibody was verified by blocking with a phospho- or non-phosphopeptide and by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer. | |
Western blot分析新生小鼠和胎大鼠大脑提取物，使用的抗体是Phospho-Doublecortin (Ser334) Antibody 兔多抗。抗体的磷酸化特异性由磷酸化或非磷酸化肽段阻断，在western转膜后对膜进行(+)或不进行(-)小牛肠碱性磷酸酶（CIP）处理。 |
