Phospho-Doublecortin (Ser297) Antibody
货号：
4605S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4605S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Doublecortin (Ser297) Antibody detects endogenous levels of doublecortin only when phosphorylated at Ser297. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
参考图片Western blot analysis of extracts from fetal rat and fetal human brain using Phospho-Doublecortin (Ser297) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer. Western blot分析胎大鼠和人胚胎大脑提取物，使用的抗体是Phospho-Doublecortin (Ser297) Antibody 兔多抗。抗体的磷酸化特异性由western转膜后对膜进行(+)或不进行(-)小牛肠碱性磷酸酶（CIP）处理验证。 |
