| 货号 | 4880S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Phospho-DBC1 (Thr454) Antibody detects endogenous levels of DBC1 only when phosphorylated on Thr454. |
| 使用方法 | WB(1:1000) IP (1:25) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Deleted in breast cancer gene 1 protein (DBC1) was originally identified by its localization to a region of chromosome 8p21 that is homozygously deleted in breast cancer (1). DBC1 is a large, nuclear protein with multiple functions in cell survival. It binds directly to the estrogen receptor α (ERα) hormone-binding domain in a ligand-independent manner and may be a key determinant of ligand-independent ERα expression and survival in human breast cancer cells (2). DBC1 can promote p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity (3). DBC1 may be an important regulator of heterochromatin formation as it binds SUV39H1 and inhibits its histone methyltransferase activity (4). Caspase-dependent processing activates the pro-apoptotic activity of DBC1 during Tumor Necrosis Factor-α (TNF-α)-mediated cell death signaling (5). This processing of DBC1 in response to TNF-α is an early event in the onset of apoptosis and results in relocalization of DBC1 to the cytoplasm. Overexpression of the processed, cytoplasmic form of DBC1 results in mitochondrial clustering and matrix condensation and sensitizes cells to TNF-α-mediated apoptosis. |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) and UV-treated (right), using Phospho-DBC1 (Thr454) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用Phospho-DBC1 (Thr454) Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞，细胞分为untreated (左图)和UV-treated (右图)。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from 293 and HeLa cell lines, untreated or UV-treated (100 J/m2 and 4 hour recovery), using Phospho-DBC1 (Thr454) Antibody (upper) or β-actin Antibody #4967 (lower). 使用Phospho-DBC1 (Thr454) Antibody (上图)或β-actin Antibody #4967 (下图)，免疫印迹(Western blot)分析293和HeLa细胞中Phospho-DBC1的蛋白水平，细胞分为untreated或UV-treated (100 J/m2和4 hour recovery)。 |
