Phospho-DARPP-32 (Thr75) Antibody
货号：
2301S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2301S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-DARPP-32 (Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75. The antibody does not cross-react with DARPP-32 phosphorylated at Thr34. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype (3). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
参考图片Western blot analysis of extracts from rat brain cortex, untreated or phosphorylated in vitro by PKA, MAP kinase or cdc2 kinase, using Phospho-DARPP-32 (Thr75) Antibody #2301 (top), Phospho-DARPP-32 (Thr34) Antibody (middle) or DARPP-32 Antibody #2302 (bottom). Western blot分析大鼠大脑皮层（未处理，或PKA、MAP激酶和cdc2激酶体内磷酸化处理），使用的抗体是Phospho-DARPP-32 (Thr75) Antibody 兔多抗（上图），Phospho-DARPP-32 (Thr34) Antibody 兔多抗（中图）或DARPP-32 Antibody 兔多抗#2302（下图）。 |
