Phospho-Dab1 (Tyr220) Antibody
货号：
3327S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3327S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Dab1 (Tyr220) Antibody detects transfected levels of Dab1 protein only when phosphorylated at tyrosine 220. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Reelin signaling pathway plays a critical role in neuronal development. Reelin is a secreted glycoprotein that binds to the lipoprotein receptors VLDLR and ApoER2 or alpha3beta1 integrin on the surface of neurons (1,2). Activation of these receptors induces tyrosine phosphorylation of Disabled 1 (Dab1), an intracellular adaptor. It is generally believed that tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is the most critical downstream event in Reelin signaling. The phosphotyrosine-binding (PTB) domain within its amino terminus enables Dab1 to recognize and bind to a conserved sequence motif within the cytoplasmic tail of the receptors. In addition, the PTB contains a Pleckstrin Homology-like subdomain that binds to phosphoinositides. The phosphoinositide-binding region within the Dab1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab1 independent of VLDLR and ApoER2 (3). It has been demonstrated that Src, CrkII, CrkL and Dock1 associate with tyrosine-phosphorylated Dab. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232 (4). |
| 存放说明 | -20C |
| 计算分子量 | 80, 110 GFP-Dab1 fusion. |
参考图片Western blot analysis of cell lysates from COS cells co-transfected with GFP-Dab1 and Fyn expression constructs, untreated and treated with calf intestinal phosphatase (CIP), using Phospho-Dab1 (Tyr220) Antibody. Western blot分析共转染GFP-Dab1和Fyn表达载体的COS细胞提取物，未处理或用小牛肠碱性磷酸酶（CIP）处理，使用的抗体是Phospho-Dab1 (yr220) Antibody 兔多抗。 |
