Phospho-CrkII (Tyr221) Antibody
货号：
3491S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3491S |
| 反应种属 | Human/Hamster |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | CrkII, a cellular homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases (1). The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk (2). The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mammalian CrkII proteins (3). Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase (4), IGF-I receptor (5) and EGF receptor (6). Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecular folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex (3).CrkII蛋白是一个细胞内v-Crk的同系物，该蛋白属于含有SH2-SH3-SH3结构域的受体蛋白家族，上述结构域能传递来自酪氨酸激酶的信号(1)。Crk蛋白的主要功能是通过SH2-磷酸化-酪氨酸的相互作用招募在酪氨酸激酶附近的细胞质蛋白。因此，来自Crk蛋白的输出取决于SH3结合蛋白，这包括C3G和Sos鸟嘌呤核苷酸交换蛋白、Abl酪氨酸激酶、DOCK180和一些 STE20相关激酶。Crk结合蛋白的种类表明Crk蛋白的多种功能(2)。CrkII蛋白两个 SH3结构域通过一个54氨基酸连接区被分开，这在爪蟾属、小鸡和哺乳类CrkII蛋白中高度保守(3)。在这个区域Tyrosine 221位点通过Abl酪氨酸激酶(4)、IGF-I受体(5)和EGF受体(6)磷酸化。一旦Tyr221位点被磷酸化，CrkII蛋白在分子内折叠和SH2-pTyr 相互作用方面有一个改变，这会引起CrkII蛋白从酪氨酸激酶复合物上快速分离(3)。 |
| 存放说明 | -20C |
| 计算分子量 | 42 |
参考图片Western blot analysis of extracts from K562 cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-CrkII (Tyr221) Antibody.使用Phospho-CrkII (Tyr221) Antibody，免疫印迹（Western Blot）分析K562细胞系中Phospho-CrkII (Tyr221)蛋白水平，细胞分为未处理组和calf intestinal phosphatase (CIP)处理组。 |
