Phospho-Cortactin (Tyr421) Antibody
货号：
4569S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4569S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Cortactin (Tyr421) Antibody detects endogenous levels of cortactin protein only when phosphorylated at Tyr421. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Cortactin is a cortical actin binding protein. Its amino-terminal acidic domain (NTA) associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3 (1,2). Cortactin is involved in signaling events that coordinate actin reorganization during cell movement. The human cortactin homologue EMS1 is overexpressed in numerous cancers with poor patient prognosis (3). Cortactin may also play an important role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network (4).
Cortactin is phosphorylated at tyrosine residues 421, 466, and 482. Tyrosine phosphorylation of cortactin regulates cell motility (5), rac1-mediated actin dynamics (6), cadherin-dependent adhesion (7), chemokine trafficking and chemokine-dependent chemotaxis (8). |
| 存放说明 | -20C |
| 计算分子量 | 80-85 |
参考图片Western blot analysis of extracts from HeLa or NIH/3T3 cells, untreated or treated with hydrogen peroxide, using Phospho-Cortactin (Tyr421) Antibody in the presence of no peptide (A), a phospho-cortactin (Tyr421) peptide (B) or a nonphospho-cortactin peptide (C). 使用Phospho-Cortactin (Tyr421) Antibody，其抗体分别孵育no peptide (A)、a phospho-cortactin (Tyr421) peptide (B)或a nonphospho-cortactin peptide (C)，免疫印迹（Western Blot）分析HeLa和NIH/3T3细胞系中Phospho-Cortactin (Tyr421)蛋白水平，细胞分为未处理组和hydrogen peroxide处理组。 |
