| 货号 | 13111S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | CUB domain containing protein 1 (CDCP1, SIMA135) is a putative stem cell marker shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreasedCDCP1 expression (2). The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. CDCP1 has three extracellular CUB domains that may be involved in cell adhesion or extracellular matrix interactions (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins (3). CDCP1 is a putative hematopoietic stem cell marker (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Western blot analysis of extracts of ZR-75-1 cells, untreated (-) or treated with a Cub1 antibody (+), using Phospho-CDCP1 (Tyr707) Antibody (upper) and CDCP1 Antibody #4115 (lower).未处理(-)或经过Cub1抗体处理(+)的ZR-75-1细胞提取物，使用Phospho-CDCP1 (Tyr707) Antibody (上)和 CDCP1 Antibody #4115 (下)进行western blot分析。 | |
Immunoprecipitation of Phospho-CDCP1 (Tyr707) protein from ZR-75-1 cell extracts, treated with a Cub1 antibody, using Normal Rabbit IgG #2729 (lane 2) or Phospho-CDCP1 (Tyr707) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-CDCP1 (Tyr707) Antibody.使用Normal Rabbit IgG #2729 (lane 2) 或 Phospho-CDCP1 (Tyr707) Antibody (lane 3)对经过Cub1抗体处理的ZR-75-1细胞提取物中的Phospho-CDCP1 (Tyr707)蛋白进行免疫沉淀实验。Lane1是10%上样量。使用Phospho-CDCP1 (Tyr707) Antibody进行western blot分析。 |
