Phospho-cdc2 (Thr161) Antibody
货号：
9114S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 9114S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).cdc2蛋白激酶的激活参与调节真核细胞进入有丝分裂，cdc2的激活受多个步骤的调控，包括周期蛋白的结合、cdc2苏氨酸161位的磷酸化(1)。然而，在细胞进入有丝分裂的过程中，激活cdc2的决定性步骤是苏氨酸14位和15位的去磷酸化(2)。Wee1 和 Myt1蛋白激酶通过磷酸化cdc2苏氨酸14位和15位，抑制cdc2活性(3,4)。而cdc25磷酸酯酶可能起到使cdc2苏氨酸14位和15位去磷酸化从而激活cdc2的作用(1,5)。 |
| 存放说明 | -20C |
| 计算分子量 | 34 |
参考图片Western blot analysis of cdc2 kinase treated with lambda protein phosphatase (lambda-PPase) for the indicated times, using Phospho-cdc2 (Thr161) Antibody.western blot 方法检测特定时间点用lambda蛋白磷酸酶(lambda-PPase) 处理cdc2激酶，使用的抗体为Phospho-cdc2 (Thr161) Antibody。 |
Western blot analysis of extracts from HeLa cells synchronized in G0, G1/S, or M phase, using Phospho-cdc2 (Thr161) Antibodywestern blot方法检测在G0、G1/S或M期同步化的HeLa细胞提取物，使用的抗体为Phospho-cdc2 (Thr161) Antibody。 |
