| 货号 | 2911S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Phospho-Catenin δ-1 (Tyr228) Antibody detects endogenous levels of catenin δ-1 protein only when phosphorylated at Tyr228. The antibody might cross react with another overexpressed phospho-tyrosine protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4). |
| 存放说明 | -20C |
| 计算分子量 | 95, 100 |
Western blot analysis of extracts from A431 cells, serum-starved overnight and then either left untreated or treated with EGF for 15 minutes, using Phospho-Catenin δ-1 (Tyr228) Antibody (upper) and Catenin δ-1 Antibody #4989 as a loading control (lower).血清饥饿过夜随后不加处理或使用EGF处理15分钟的A431细胞提取物，使用Phospho-Catenin δ-1 (Tyr228) 抗体（上）和作为上样对照的Catenin δ-1抗体#4989（下）进行western blot分析。 | |
Confocal immunofluorescent analysis of A431 cells, serum-starved (left) or EGF-treated (right), using Phospho-Catenin δ-1 (Tyr228) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).血清饥饿（左）或EGF处理（右）的A431细胞，使用Phospho-Catenin δ-1 (Tyr228) 抗体（绿色）进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5™ （DNA荧光染料）。 |
