Phospho-CARD11 (Ser652) Antibody
货号：
5189S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 5189S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-CARD11 (Ser652) Antibody detects endogenous levels of CARD11 protein only when phosphorylated at Ser652. This site corresponds to Ser671 in mouse and Ser668 in rat CARD11 proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family that typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
参考图片Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #9905 (40 nM) and calcium ionophore A23187 (2 μM) for 15 minutes, and λ-phosphatase plus calf intestinal phosphatase (CIP) as indicated, using Phospho-CARD11 (Ser652) Antibody (upper) and CARD11 (1D12) Rabbit mAb #4435 (lower).Western免疫印迹。用Phospho-CARD11 (Ser652) Antibody (上图) 和CARD11 (1D12) Rabbit mAb #4435 (下图)研究未经处理的和经 TPA #9905 (40 nM) ，钙离子载体A23187 (2 μM) 15 min, λ-磷酸酶和小牛碱性磷酸酶(CIP)处理的Jurkat细胞的细胞提取液。 |
