Phospho-CAD (Ser1859) Antibody
货号：
12662S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 12662S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-CAD (Ser1859) Antibody recognizes endogenous levels of CAD protein only when phosphorylated at Ser1859. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | CAD is essential for the de novo synthesis of pyrimidine nucleotides and possesses the following enzymatic activities: glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase. Thus, the enzyme converts glutamine to uridine monophosphate, a common precursor of all pyrimidine bases, and it is necessary for nucleic acid synthesis (1). In resting cells, CAD is localized mainly in the cytoplasm where it carries out pyrimidine synthesis. As proliferating cells enter S phase, MAP Kinase (Erk1/2) phosphorlyates CAD at Thr456, resulting in CAD translocation to the nucleus. As cells exit S phase, CAD is dephosphorylated at Thr456 and phosphorylated at Ser1406 by PKA, returning the pathway to basal activity (2). Various research studies have shown increased expression of CAD in several types of cancer, prompting the development of pharmacological inhibitors such as PALA. Further studies have identified CAD as a potential predictive early marker of prostate cancer relapse (3). |
| 存放说明 | -20C |
| 计算分子量 | 240 |
参考图片Western blot analysis of extracts from NIH/3T3 cells untreated (-) and HeLa cells, untreated (-) or treated with Rapamycin #9904 (10 μM, 16 hr; +), using Phospho-CAD (Ser1859) Antibody (upper) or CAD Antibody #11933 (lower). Western blot 检测未处理NIH/3T3细胞系提取物(-) 和未处理(-) 或Rapamycin #9904(10 μM, 16 hr; +)处理的 HeLa cells，使用抗体为Phospho-CAD (Ser1859) Antibody (上图) 或 CAD Antibody #11933 (下图)。 |
