| 货号 | 2841S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-C/EBPalpha (Ser21) Antibody detects endogenous levels of C/EBPalpha only when phosphorylated at serine 21. This antibody does not cross-react with other phosphorylated C/EBP isoforms. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-1 stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226 and Ser230 by GSK3 seems to be required for adipogenesis (4). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from mouse adipocytes treated with insulin for the indicated times, using Phospho-C/EBPalpha (Ser21) Antibody. 对小鼠脂肪细胞抽提液，胰岛素处理适当时间，使用Phospho-C/EBPalpha (Ser21)抗体进行Western blot分析。 | |
Western blot analysis of extracts of COS cells untransfected (lane 1), or transfected with wild-type mouse C/EBPalpha (lane 2), S21A (lane 3), and S21D (lane 4) mutants, using Phospho-C/EBPalpha (Ser21) Antibody (upper) or C/EBPalpha Antibody (lower). (Provided by Dr. Hanna Radomska, Beth Israel Deaconess Medical Center, Boston, MA). 对COS细胞，未转染（列1）或转染野生型小鼠C/EBPalpha（列2）、S21A（列3）、S21D （列4）突变体，使用Phospho-C/EBPalpha (Ser21)Antibody（上图）或C/EBPalpha Antibody（下图）进行Western blot分析。（抗体由Dr. Hanna Radomska, Beth Israel Deaconess Medical Center, Boston, MA提供） |
