| 货号 | 13936S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Circadian rhythms govern many key physiological processes that fluctuate with a period of approximately 24 hours. These processes include the sleep-wake cycle, glucose, lipid and drug metabolism, heart rate, hormone secretion, renal blood flow, and body temperature, as well as basic cellular processes such as DNA repair and the timing of the cell division cycle (1,2). The mammalian circadian system consists of many individual tissue-specific clocks (peripheral clocks) that are controlled by a master circadian pacemaker residing in the suprachiasmatic nuclei (SCN) of the brain (1,2). The periodic circadian rhythm is prominently manifested by the light-dark cycle, which is sensed by the visual system and processed by the SCN. The SCN processes the light-dark information and synchronizes peripheral clocks through neural and humoral output signals (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from mouse brain and rat eye using Phospho-BMAL1 (Ser42) Antibody. The phospho-specificity of Phospho-BMAL1 (Ser42) Antibody was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Ser42. | |
Western blot analysis of extracts from mouse brain, untreated (-) or λ phosphatase-treated (+), using Phospho-BMAL1 (Ser42) Antibody (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full length wild-type mouse BMAL1 protein (wt mBMAL1-DDK, +) or DDK-tagged full length mouse BMAL1 Ser42A site specific mutant (S42A mBMAL1-DDK; +), using Phospho-BMAL1 (Ser42) Antibody (upper) and DYKDDDDK Tag (9A3) Mouse mAb #8146 (lower). Wild-type and S42A site specific mutant BMAL1 constructs were kindly provided by Dr. Jonathan Lipton (Boston Childrens Hospital). |
