| 货号 | 13325S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-BCL9L (Ser915) Antibody recognizes endogenous levels of BCL9L protein only when phosphorylated at Ser915. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Phospho-BCL9L (Ser915) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CSTs LC-MS/MS platform for modification site discovery. Phosphorylation at Ser915 was discovered using an AMPK substrate antibody. Please visit PhosphoSitePlus®, CSTs modification site knowledgebase, at www.phosphosite.org for more information.此Phospho-BCL9L (Ser915)抗体是由于Cell Signaling Technology (CST)使用PhosphoScan®和 CSTs LC-MS/MS 平台的发现蛋白修饰点而设计的。Ser915的磷酸化是通过一个AMPK底物抗体发现的，并在多种细胞系中发现可以被胰岛素刺激诱导表达。请访问www.phosphosite.org上的CSTs修饰位点网站－PhosphoSitePlus®，以获得更多的信息。 |
| 存放说明 | -20C |
| 计算分子量 | 200 |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (100 μM, 2 hr; +), using Phospho-BCL9L (Ser915) Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). 未处理(-)或使用carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (100 μM, 2 hr; +)处理的MCF7细胞并获取细胞提取物，使用Phospho-BCL9L (Ser915) 抗体 (上)或 β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下)进行western blot分析。 | |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with TPA #4174 (200 nM, 30 min; +), using Phospho-BCL9L (Ser915) Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). 未处理(-)或使用TPA #4174 (200 nM, 30 min; +)处理MCF7细胞并获取提取物，使用Phospho-BCL9L (Ser915) 抗体 (上)或 β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下)进行western blot分析。 |
