Phospho-Bad (Ser155) Antibody
货号：
9297S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 9297S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Bad (Ser155) Antibody detects transfected levels of Bad only when phosphorylated at Ser155. This antibody does not detect Bad phosphorylated at other sites or related family members. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
参考图片Western blot analysis of cell extracts from 293 cells transfected with Wild-type Bad, Bad (S112A/S136A), Bad (S155A) and treated with forskolin (30 µM), using Phospho-Bad (Ser155) Antibody (upper) and Bad Antibody #9292 (lower). Western blot 免疫印记分析转染了野生型Bad、Bad (S112A/S136A)、Bad (S155A) 并经过forskolin (30 µM) 处理的293细胞的细胞提取液。用Phospho-Bad (Ser155) Antibody (上图) 和Bad Antibody #9292 (下图)。 |
