| 货号 | 9295S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Bad (Ser136) Antibody detects transfected levels of Bad only when phosphorylated at Ser136. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members. |
| 使用方法 | WB(1:500) |
| 供应商 | CST |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (S112A), Bad (S136A) or Bad (S112A/S136A), untreated, TPA-treated or forskolin-treated, using Phospho-Bad (Ser112) Antibody #9291 (top), Phospho-Bad (Ser136) Antibody (middle) or Bad Antibody #9292 (bottom). Western blot（免疫印迹）分析分别转染了野生型Bad、Bad (Ser112A)、Bad (S136A)、Bad (S112A/S136A)并经TPA或者forskolin处理的或者未经处理的 293 细胞的细胞提取液，用Phospho-Bad (Ser112) Antibody #9291 (上图)，Phospho-Bad (Ser136) Antibody (中间图) or Bad Antibody #9292 (下图)研究。 | |
Western blot analysis of GST-Bad, phosphorylated by CKII or PKA in vitro, using Phospho-Bad (Ser112) Antibody #9291 (top), Phospho-Bad (Ser136) Antibody (middle) or Bad Antibody #9292 (bottom). Western免疫印迹。用Phospho-Bad (Ser112) Antibody #9291 (上图), Phospho-Bad (Ser136) Antibody (中间图) or Bad Antibody #9292 (下图)研究被CKII或PKA体外磷酸化的GST-Bad蛋白。 |
