| 货号 | 11983S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | BACH1, also known as BRIP1 and FANCJ, is a DNA helicase involved in repair of DNA cross-links and double strand breaks (1-3). Interaction between phosphorylated BACH1 and BRCA1 is required for DNA damage-induced checkpoint signaling (3,4). Originally identified as a breast cancer susceptibility gene (1), the BACH1 gene is mutated in Fanconi anemia (5), a recessive disorder characterized by multiple congenital abnormalities, progressive bone marrow failure, and high cancer risk/predisposition. Research investigators have concluded that BACH1 interactions with BRCA1 and the presence of BACH1 mutations in patients with early onset breast cancer indicate that BACH1 may act as a tumor suppressor (6). Phosphorylation of BACH1 at Thr1133 is thought to be involved in regulation of the replication checkpoint and is required for the interaction of BACH1 with TopBP1 (7). BACH1，也被称为BRIP1 和FANCJ，是一种涉及DNA交联和双链损伤修复的DNA解旋酶（1-3）。磷酸化的的BACH1和BRCA1相互作用对于DNA损伤引发的检验点信号通路是必须的（3,4）。BACH1最初被认为是一种乳腺癌敏感基因（1），范科尼贫血病人体内的BRCA1基因会发生突变（5），范科尼贫血是一种隐性遗传的疾病，其特征包括多发性先天异常，渐进的骨髓造血功能衰竭，和癌症高风险/倾向。研究人员已经得出结论BACH1与BRCA1结合，以及在早发乳腺癌病人体内发现的BACH1突变表明BACH1可能作为一种抑癌因子存在（6）。BACH1的1133位苏氨酸磷酸化被认为涉及复制检验点调控和BACH1与TopBP1的相互作用（7）。 |
| 存放说明 | -20C |
| 计算分子量 | 145 |
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with λ phosphatase and calf intestinal phosphatase (CIP) (+), using Phospho-BACH1/BRIP1 (Thr1133) Antibody (upper) or BACH1/BRIP1 Antibody #4578 (lower).使用Phospho-BACH1/BRIP1 (Thr1133) 抗体(上)或 BACH1/BRIP1 抗体 #4578 (下)未处理(-)或使用λ phosphatase和牛小肠碱性磷酸酶(CIP) (+)处理的Jurkat细胞提取物进行western blot分析。 | |
Immunoprecipitation of phospho-BACH1/BRIP1 (Thr1133) from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-BACH1/BRIP1 (Thr1133) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-BACH1/BRIP1 (Thr1133) Antibody.使用正常Rabbit IgG#2729(lane 2)或Phospho-BACH1/BRIP1 (Thr1133) 抗体(lane 3)对293T细胞提取物的磷酸化BACH1/BRIP1 (Thr1133)进行免疫沉淀实验。Lane1使用10%上样量。Phospho-BACH1/BRIP1 (Thr1133)抗体也用于western blot实验。 |
