Phospho-ASK1 (Thr845) Antibody
货号：
3765S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3765S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Apoptosis signal-regulating kinase 1 (ASK1), a MAP kinase kinase kinase, plays essential roles in stress-induced apoptosis (1,2). ASK1 is activated in response to a variety of stress-related stimuli through distinct mechanisms and activates MKK4 and MKK3, which in turn activate JNK and p38 (3). Overexpression of ASK1 activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation and cell death (4,5). Phosphorylation at Ser967 is essential for ASK1 association with 14-3-3 protein and suppression of cell death (6). Oxidative stress induces dephosphorylation of Ser967 and phosphorylation of Thr845 in the activation loop of ASK1, and both are correlated with ASK1 activity and ASK1-dependent apoptosis (7,8). On the other hand, Akt phosphorylates ASK1 at Ser83, which attenuates ASK1 activity and promotes cell survival (9). |
| 存放说明 | -20C |
| 计算分子量 | 155 |
参考图片Western blot analysis of extracts from HEK293 cells, untransfected (lane 1) or transfected with wild-type ASK1 (lane 2) or T845A mutant ASK1 (lane 3), using Phospho-ASK1 (Thr845) Antibody (upper) or ASK1 Antibody #3762 (lower). (Lysates were provided by Dr. Wang Min, Dept. of Pathology, Yale University, New Haven, CT).western blot方法检测细胞提取物：未转染的（1、4号条带）、转染过原株ASK1蛋白的（2号条带）、转染Ser83Ala 突变ASK1蛋白的（3号条带）HEK293细胞，使用的抗体是Phospho-ASK1 (Thr845) Antibody (上图) 和 ASK1 Antibody #3762 (下图)。 |
Western blot analysis of extracts from COS cells, untransfected (lane 1), or transfected with wild-type ASK1 (lanes 2,3), using Phospho-ASK1 (Thr845) Antibody (upper) or ASK1 Antibody #3762 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of Phospho-ASK1 (Thr845) Antibody.western blot方法检测细胞提取物：未转染的（1号条带）、转染过原株ASK1蛋白的（2、3号条带）COS细胞，使用的抗体是Phospho-ASK1 (Thr845) Antibody (上图) 和 ASK1 Antibody #3762 (下图)。3号条带中细胞裂解物用lamda 磷酸酶处理，以证明Phospho-ASK1 (Thr845) 抗体具有磷酸化特异性。 |
