Phospho-ASK1 (Ser967) Antibody
货号：
3764S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3764S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Apoptosis signal-regulating kinase 1 (ASK1), a MAP kinase kinase kinase, plays essential roles in stress-induced apoptosis (1,2). ASK1 is activated in response to a variety of stress-related stimuli through distinct mechanisms and activates MKK4 and MKK3, which in turn activate JNK and p38 (3). Overexpression of ASK1 activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation and cell death (4,5). Phosphorylation at Ser967 is essential for ASK1 association with 14-3-3 protein and suppression of cell death (6). Oxidative stress induces dephosphorylation of Ser967 and phosphorylation of Thr845 in the activation loop of ASK1, and both are correlated with ASK1 activity and ASK1-dependent apoptosis (7,8). On the other hand, Akt phosphorylates ASK1 at Ser83, which attenuates ASK1 activity and promotes cell survival (9). |
| 存放说明 | -20C |
| 计算分子量 | 155 |
参考图片Western blot analysis of extracts from COS7 cells transfected with wild-type or Ser967Ala mutant ASK1, using Phospho-ASK1 (Ser967) Antibody (A,B) or ASK1 Antibody #3762 (C,D). The phospho-specificity of the antibody was further characterized by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer (B,D).western blot方法检测细胞提取物：将原株ASK1蛋白和含有Ser83Ala 突变的ASK1蛋白转染至COS7细胞，使用的抗体是Phospho-ASK1 (Ser967) Antibody (A,B图)和 ASK1 Antibody #3762 (C,D图)。电转移后用牛小肠碱性磷酸酶（CIP）处理转印膜（B、D图所示）使该抗体的磷酸化特异性加强。 |
