Phospho-AS160 (Thr642) Antibody
货号：
4288S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4288S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-AS160 (Thr642) Antibody detects endogenous levels of AS160 protein only when phosphorylated at Thr642. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The polypeptide insulin is a major hormone controlling critical energy functions such as glucose and lipid metabolism. Insulin binds to and activates the insulin receptor (IR) tyrosine kinase, which phosphorylates and recruits different adaptor proteins. The signaling pathway initiated by insulin and its receptor stimulates glucose uptake in muscle cells and adipocytes through translocation of GLUT4 glucose transporter from the cytoplasm to the plasma membrane (1). A 160 kDa substrate of the Akt Ser/Thr kinase (AS160, TBC1D4) is a Rab GTPase-activating protein that regulates insulin-stimulated GLUT4 trafficking. AS160 is expressed in many tissues including brain, kidney, liver, and brown and white fat (2). Multiple Akt phosphorylation sites have been identified on AS160 in vivo, with five sites (Ser318, Ser570, Ser588, Thr642, and Thr751) showing increased phosphorylation following insulin treatment (2,3). Studies using recombinant AS160 demonstrate that insulin-stimulated phosphorylation of AS160 is a crucial step in GLUT4 translocation (3) and is reduced in some patients with type 2 diabetes (4). The interaction of 14-3-3 regulatory proteins with AS160 phosphorylated at Thr642 is a necessary step for GLUT4 translocation (5). Phosphorylation of AS160 by AMPK is involved in the regulation of contraction-stimulated GLUT4 translocation (6). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
参考图片Western blot analysis of extracts from serum starved HeLa cells, untreated or treated with either insulin (150 nM, 15 minutes) or IGF-1 #3093 (100 ng/ml, 15 minutes), using Phospho-AS160 (Thr642) Antibody (upper) or AS160 (C69A7) Rabbit mAb #2670 (lower). The phospho-specificity of the antibody is verified by λ-phosphatase treatment of the above cell lysates. Wetern blot检测血清饥饿的Hela细胞提取物，未处理或用胰岛素和IGF-1处理，所用抗体为Phospho-AS160 (Thr642) Antibody (上)和AS160 (C69A7) Rabbit mAb兔单抗 #2670 (下)。抗体的磷酸化特异性通过λ磷酸酶处理来验证。 |
