| 货号 | 3823S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-APP (Thr668) Antibody detects different isoforms of endogenous amyloid β (A4) precursor protein only when phosphorylated at Thr668 (or the corresponding position on other isoforms). |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Amyloid β (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-β), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-β fragments form the main components of amyloid plaques in Alzheimer disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent and peaks during G2/M-phase (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 100-140 |
Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated, and untransfected or transfected with APP, using Phospho-APP (Thr668) Antibody (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower). Western blot分析HeLa细胞（未处理或nocodazole处理），未转染和转染APP，使用的抗体是Phospho-APP (Thr668) Antibody 兔多抗（上图）或Akt (pan) (C67E7) Rabbit mAb 兔单抗 #4691 （下图）。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or λ-phosphatase treated (right), using Phospho-APP (Thr668) Antibody (green), β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (blue). 共聚焦免疫荧光分析Hela细胞，未处理（左），λ-磷酸化（右），使用Phospho-APP (Thr668) Antibody 兔多抗（绿），β-Tubulin (9F3) Rabbit mAb 兔单抗 (Alexa Fluor®555 Conjugate) #2116（红）和 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb 鼠单抗 #9706 （蓝）进行标记。 |
