Phospho-AP2M1 (Thr156) Antibody
货号：
3843S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3843S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-AP2M1 (Thr156) Antibody detects endogenous levels of AP2M1 protein only when phosphorylated at Thr156. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The AP-2 coat assembly protein complex is an important component of clathrin coat pits involved in receptor-mediated endocytosis at the plasma membrane (1-3). Each AP-2 heterotetramer is composed of α, β, μ and σ protein subunits. The 50 kDa μ subunit (AP-2μ, AP2M1) is located at the core of the AP-2 complex and mediates the interaction between the cargo protein and the clathrin coated pit (1-4). The carboxy-terminal AP2M1 region recognizes the tyrosine-based, endocytotic sorting motif YXXφ found in cargo proteins and helps to bring the cargo protein to the clathrin-coated pit. Non-canonical, tyrosine-based endocytotic sorting signals can also promote interaction between cargo proteins and AP2M1 (5,6). AP2M1 plays an essential role in molecular signaling as it couples receptor-mediated endocytosis and pathways involving membrane receptors (7-9), matrix metalloproteinases (10) and ion channel proteins (11). Phosphorylation of specific AP2M1 residues and binding of lipids to this adaptor protein can regulate AP2M1 activity (12,13). Phosphorylation of AP2M1 at Thr156 by adaptor-associated kinase 1 (AAK1) stimulates affinity binding of AP2M1 to cargo protein signals (14). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
参考图片Western blot analysis of extracts from HeLa cells, serum-starved overnight and either untreated or H2O2-treated (4 mM, 30 minutes), using Phospho-AP2M1 (Thr156) Antibody (upper) or an AP2M1 rabbit mAb (lower). Western blot 免疫印记。用 Phospho-AP2M1 (Thr156) Antibody (上图) 或者AP2M1 rabbit mAb 兔单抗 (下图)研究血清饥饿过夜，并未经处理或经过H2O2(4mM, 30分钟)处理的HeLa细胞的细胞提取液。 |
