Phospho-AMPA Receptor 2 (GluA2) (Tyr876) Antibody
货号：
4027S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4027S |
| 反应种属 | Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody detects endogenous levels of GluR 2 only when phosphorylated at Tyr876. It may also detect GluR 3 when phosphorylated at the conserved Tyr887. This residue is not conserved in GluR 1 or GluR 4. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2 containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Activity changes of AMPARs are implicated in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).Src family tyrosine kinases phosphorylate the GluR 2 subunit of AMPA receptors at Tyr876, which increases the interaction with GRIP1/2 but not PICK1. In addition, Tyr876 is important for AMPA- and NMDA-induced GluR 2 internalization (3).
The phosphorylation site at Tyr876 was also independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CSTs MS/MS platform for phosphorylation site discovery. Phosphorylation of GluR at Tyr876 was observed in extracts isolated from ischemic rat brain. |
| 存放说明 | -20C |
| 计算分子量 | 100 |
参考图片Western blot analysis of extracts from rat brain, either sham-operated, 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody (upper) or AMPA Receptor (GluR 2/3/4) Antibody #2460 (lower). Western blot分析大鼠大脑（假手术组、15分钟缺血后再灌注4小时或仅15分钟缺血），使用的抗体是Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody 兔多抗 （上图）或AMPA Receptor (GluR 2/3/4) Antibody 兔多抗#2460（下图）。 |
