| 货号 | 9601S |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/E-P |
| 目标/特异性 | Phospho-(Ser) 14-3-3 Binding Motif Antibody binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. The antibody weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif, and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding nonphosphorylated sequences or with other phospho-Thr/Ser/Tyr-containing motifs. By ELISA this antibody recognizes a wide range of peptides containing the 14-3-3 binding motif, and by 2D gel Western blot analysis it recognizes a large number of presumptive 14-3-3 binding proteins. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) E-P (1:1000) |
| 供应商 | CST |
| 背景 | The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions (6). Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins (7). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25C, FKHRL1, PKC and c-Raf (5,7). Phospho-(Ser) 14-3-3 Binding Motif Polyclonal and (4E2) Monoclonal Antibodies provide powerful tools for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery. |
| 存放说明 | -20C |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phosphorylated 14-3-3 binding motifs, using Phospho-(Ser) 14-3-3 Binding Motif Antibody. 对石蜡包埋的人乳腺癌使用Phospho-(Ser) 14-3-3 Binding Motif Antibody进行免疫组化分析，显示包含磷酸化14-3-3结合基序的蛋白染色。 | |
Western blot analysis of extracts from 293 cells transfected with Bad or Bad Ser112 and/or Ser136 mutant fusion proteins, using Phospho-(Ser) 14-3-3 Binding Motif Antibody (top), Phospho-Bad (Ser136) Antibody #9295 (middle) or Phospho-Bad (Ser112) Antibody #9291 (bottom). 对293细胞（转染Bad或Bad Ser112和/或Ser136突变融合蛋白），使用Phospho-(Ser) 14-3-3 Binding Motif Antibody（上图），Phospho-Bad (Ser136) Antibody #9295（中图）或Phospho-Bad (Ser112) Antibody #9291（下图）进行Western blot分析。 | |
Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 30 minutes), using Phospho-(Ser) 14-3-3 Binding Motif Antibody. Proteins were separated by 2-D electrophoresis prior to blotting. 对未处理或0.1uM calyculin A裂解前处理30分钟的Jurkat细胞抽提液使用Phospho-(Ser) 14-3-3 Binding Motif Antibody进行Western blot分析。蛋白质在印记前在二维电泳分离。 | |
Phospho-(Ser) 14-3-3 Binding Motif Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (S* denotes phosphorylated serine.) Phospho-(Ser) 14-3-3 Binding Motif Antibody ELISA分析：磷酸化和非磷酸化14-3-3结合基序的信噪比。（S* 代表磷酸化的丝氨酸） |
