Methyl-NF-κB p65 (Lys310) Antibody
货号：
13188S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 13188S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
参考图片Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human NF-κB p65 (hNF-κB p65-Myc/DDK; +) and Myc/DDK-tagged full-length human SETD6 (hSETD6-Myc/DDK; +), using Methyl-NF-κB p65 (Lys310) Antibody (upper) or DYKDDDDK Tag Antibody #2368 (lower). |
Western blot analysis of extracts from HT-1080 and OVCAR8 cell lines using Methyl-NF-κB p65 (Lys310) Antibody. Antibodies were competed with peptides against methyl-NF-κB p65 (Lys310) (middle) or non-methyl-NF-κB p65 (Lys310) (right). |
