| 货号 | 9759S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Di-Methyl-Histone H4 (Lys20) Antibody detects endogenous levels of histone H4 only when di-methylated on Lys20. The antibody does not cross-react with non-, mono- or tri-methylated Lys20. In addition, the antibody does not cross-react with mono-, di- or tri-methylated histone H3 at Lys4, Lys9, Lys27 or Lys36. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9). |
| 存放说明 | -20C |
| 计算分子量 | 11 |
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H4 (Lys20) Antibody. 使用Di-Methyl-Histone H4 (Lys20) Antibody，免疫印迹(Western blot)分析不同细胞中Di-Methyl-Histone H4 (Lys20)蛋白水平。 | |
Di-Methyl-Histone H4 (Lys20) Antibody specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated di-methyl histone H4 (Lys20) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the di-methyl histone H4 (Lys20) peptide competed away binding of the antibody. 通过peptide ELISA确定Di-Methyl-Histone H4 (Lys20) Antibody的特异性。该图描述了抗体与提前包被的di-methyl histone H4 (Lys20) peptide的结合能力，并且多肽中含有浓度递增的不同竞争多肽。如同所示，仅di-methyl histone H4 (Lys20) peptide竞争脱离抗体的结合。 |
