Cleaved DFF45 (Asp224) Antibody
货号：
9731S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 9731S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Cleaved DFF45 (Asp224) Antibody detects the carboxy-terminal fragment of DFF45 resulting from cleavage at aspartic acid 224. The antibody does not recognize full length DFF45 or other cleavage products of DFF45. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 12 |
参考图片Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM), using Cleaved DFF45 (Asp224) Antibody. 采用Cleaved DFF45 (Asp224) Antibody对未处理与依托泊苷处理（25uM）的Jurkat细胞进行免疫印迹分析（western blot）分析。 |
