| 货号 | 9505S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Cleaved Caspase-9 (Asp315) Antibody (Human Specific) detects endogenous levels of the 35 kDa large fragment of caspase-9 following cleavage at aspartic acid 315. The antibody does not react with full length caspase-9 or any other cleaved caspases. |
| 使用方法 | WB(1:1000) IP (1:500) |
| 供应商 | CST |
| 背景 | Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
Western blot analysis of extracts from Jurkat cells, untreated or cytochrome c-treated (0.25 mg/ml), and HeLa cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using Cleaved Caspase-9 (Asp315) Antibody (Human Specific) (upper) or Caspase-9 Antibody (Human Specific) #9502 (lower). Western blot 分析未处理或cytochrome c（细胞色素C）(0.25 mg/ml)处理的 Jurkat 细胞提取物，以及未处理或staurosporine（星胞菌素） (1 µM)或cytochrome c（细胞色素C）(0.25 mg/ml)处理的HeLa 细胞，抗体采用Cleaved Caspase-9 (Asp315) Antibody (人特异性) (上) 或 Caspase-9 Antibody #9502 (人特异性) (下)。 |
