| 货号 | 2591S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Acetyl-Histone H4 (Lys12) Antibody detects endogenous levels of histone H4 only when acetylated at Lys12. The antibody does not cross-react with other acetylated histones. |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 11 |
Western blot analysis of extracts from various cell lines untreated or TSA-treated (400 nM TSA for 12 hours) using Acetyl-Histone H4 (Lys12) Antibody. 使用Acetyl-Histone H4 (Lys12) Antibody，免疫印迹(Western blot)分析不同细胞中Acetyl-Histone H4 (Lys12)蛋白水平，细胞分为untreated或TSA-treated (400 nM TSA for 12 hours)。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TSA-treated (right), using Acetyl-Histone H4 (Lys12) Antibody (green). Actin filaments have been labled with DY-554 phalloidin (red). 使用Acetyl-Histone H4 (Lys12) Antibody (绿色)，免疫组化分析HeLa细胞石蜡切片，细胞分为untreated (左图)或TSA-treated (右图)。DY-554 phalloidin标记微丝蛋白(红色)。 |
