| 货号 | 2571S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | Acetyl-Histone H2B (Lys20) Antibody detects endogenous levels of histone H2B only when acetylated at lysine 20. The antibody does not cross-react with other acetylated histones. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1,2). The p300/CBP histone acetyltransferases acetylate multiple lysine residues in the amino terminal tail of histone H2B (Lys5, 12, 15, and 20) at gene promoters during transcriptional activation (1-3). Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the access of DNA to various DNA-binding proteins (4,5). In addition, acetylation of specific lysine residues creates docking sites that facilitate recruitment of many transcription and chromatin regulatory proteins that contain a bromodomain, which binds to acetylated lysine residues (6). Histone H2B is mono-ubiquitinated at Lys120 during transcriptional activation by the RAD6 E2 protein in conjunction with the BRE1A/BRE1B E3 ligase (also known as RNF20/RNF40) (7). Mono-ubiquitinated histone H2B Lys120 is associated with the transcribed region of active genes and stimulates transcriptional elongation by facilitating FACT-dependent chromatin remodeling (7-9). In addition, it is essential for subsequent methylation of histone H3 Lys4 and Lys79, two additional histone modifications that regulate transcriptional initiation and elongation (10). In response to metabolic stress, AMPK is recruited to responsive genes and phosphorylates histone H2B on Lys36, both at promoters and in transcribed regions of genes, and may regulate transcriptional elongation (11). In response to multiple apoptotic stimuli, histone H2B is phosphorylated at Ser14 by the Mst1 kinase (12). Upon induction of apoptosis, Mst1 is cleaved and activated by caspase-3, leading to global phosphorylation of histone H2B during chromatin condensation. Interestingly, histone H2B is rapidly phosphorylated at irradiation-induced DNA damage foci in mouse embryonic fibroblasts (13). In this case, phosphorylation at Ser14 is rapid, depends on prior phosphorylation of H2AX Ser139, and occurs in the absence of apoptosis, suggesting that Ser14 phosphorylation may have distinct roles in DNA-damage repair and apoptosis. |
| 存放说明 | -20C |
| 计算分子量 | 14 |
Immunohistochemical staining of paraffin-embedded human breast carcinoma, showing nuclear localization of histone H2B, using Acetyl-Histone H2B (Lys20) Antibody (left), or the same antibody preincubated with specific acetyl histone H2B peptide (right). 使用Acetyl-Histone H2B (Lys20) Antibody (左图)或与特异性acetyl histone H2B peptide孵育的同样抗体(右图)，免疫组化分析人源乳腺癌组织石蜡切片，结果显示histone H2B的细胞核定位。 | |
Western blot analysis of extracts from various cell lines, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H2B (Lys20) Antibody. 使用Acetyl-Histone H2B (Lys20) Antibody，免疫印迹(Western blot)分析untreated或TSA-treated (400 nM for 12 hours)的不同细胞。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Trichostatin A (TSA) #9950 (1 μM for 24 hours; right) using Acetyl-Histone H2B (Lys20) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用Acetyl-Histone H2B (Lys20) Antibody (绿色)，共聚焦免疫荧光分析HeLa细胞，细胞分为untreated (左图)或Trichostatin A (TSA) #9950 treated (1 μM for 24 hours; 右图)。DY-554 phalloidin标记微丝蛋白(红色)。 |
