| 货号 | 9441S |
| 反应种属 | Human/Mouse/Rat/S.cerevisiae/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/ChIP/E-P |
| 目标/特异性 | Acetylated-Lysine Antibody detects proteins posttranslationally modified by acetylation on the epsilon-amine groups of lysine residues. The antibody recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF and chemically acetylated BSA. The antibody has been shown to react with as little as 0.04 ng of chemically acetylated BSA while not recognizing up to 25 µg of nonacetylated BSA. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:800) IF-IC (1:100) ChIP (1:50) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10). |
| 存放说明 | -20C |
Western blot analysis of extracts from NIH/3T3 cells, untreated or sodium butyrate-treated (5 mM for 24 hours), showing an increase in histone acetylation using Acetylated-Lysine Antibody. 对NIH/3T3细胞抽提液，未处理或5 mM丁酸钠处理24小时，使用Acetylated-Lysine Antibody进行Western blot分析，显示组蛋白乙酰化增加。 | |
Western blot analysis of immunoprecipitated p53 showing an increase in p53 acetylation using Acetylated-Lysine Antibody (upper) or p53 antibody (lower). p53 was immunoprecipitated from lysates from 293 cells, untreated or UV-treated, using p53 Antibody #9282. 对免疫共沉淀的p53，使用Acetylated-Lysine Antibody（上图）或p53 antibody（下图）进行Western blot分析，显示p53乙酰化增加。从293细胞裂解后免疫共沉淀的p53，未处理或UV处理，使用p53 Antibody #9282。 | |
Immunohistochemical staining of a paraffin-embedded human breast tumor section showing nuclear and cytoplasmic localization of proteins with acetylated lysine residues using Acetylated-Lysine Antibody. 对石蜡包埋的人乳腺肿瘤切片使用Acetylated-Lysine Antibody进行免疫组化分析，显示细胞核和细胞质定位。 | |
Western blot analysis of extracts from COS cells, untreated or TSA-treated, grown in 10% FBS (lanes 1 and 2) or serum starved for 18 hours (lanes 3 and 4), using Acetylated-Lysine Antibody (upper) or p44/42 MAP Kinase Antibody #9102 (lower). 对COS细胞，未处理或TSA处理，在10% FBS（列1和列2）生长或血清饥饿处理18小时（列3和列4），使用Acetylated-Lysine Antibody（上图）或p44/42 MAP Kinase Antibody #9102（下图）进行Western blot分析。 | |
Specificity and sensitivity of Acetylated-Lysine Antibody assayed on acetylated BSA (4; 1; 0.2; 0.04 or 0.008 ng in lanes 1-5) or nonacetylated BSA (25,000; 5,000; 1,000 or 200 ng in lanes 6-9). Acetylated-Lysine Antibody的特异性或敏感性用乙酰化BSA（4、1、0.2、0.04或0.008 ng 列1-5）和非乙酰化BSA（25,000、5,000、1,000或200 ng 列6-9）进行检测。 | |
Immunohistochemical analysis of paraffin-embedded NIH/3T3 untreated (left) or TSA-treated (right) using Acetylated-Lysine Antibody. 对NIH/3T3细胞，未处理（左）或TSA处理（右），使用Acetylated-Lysine Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetylated-Lysine Antibody. 对石蜡包埋的人结肠癌使用Acetylated-Lysine Antibody抗体进行免疫组化分析。 | |
Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or SAHA-treated (right), labeled with Acetylated-Lysine Antibody (green). Actin filaments have been labeled with Alexa Fluor R 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对NIH/3T3细胞，未处理（左）或SAHA处理（右），使用Acetylated-Lysine Antibody（绿）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor® 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 20 μl of Acetylated-Lysine Antibody or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002. The enriched DNA was quantified by real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002试剂盒，将4 x 10^6 HeLa细胞的染色质，与20μl Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490和SimpleChIP® Human MYT-1 Exon 1 Primers #4493进行real-time PCR用来定量富集的DNA。每个样本中免疫沉淀的DNA使用相对于输入对照中染色质的量描述，输入对照的量相当于1。 |
