| 货号 | 98543S |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | WB,IP |
| 目标/特异性 | Phospho-Stat3 (Ser754) Antibody recognizes endogenous levels of Stat3 protein only when phosphorylated at Ser754. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). Stat3 is phosphorylated by TBK1 at Ser754 in response to cytosolic DNA reducing Stat3 transcriptional activity (11). |
| 存放说明 | -20C |
| 计算分子量 | 86 |
| 参考文献 | 1 . Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. 2 . Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4. 3 . Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15. 4 . Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85. 5 . Bromberg, J.F. et al. (1999) Cell 98, 295-303. 6 . Darnell, J.E. et al. (1994) Science 264, 1415-21. 7 . Ihle, J.N. (1995) Nature 377, 591-4. 8 . Wen, Z. et al. (1995) Cell 82, 241-50. 9 . Yokogami, K. et al. (2000) Curr Biol 10, 47-50. 10 . Biethahn, S. et al. (1999) Exp Hematol 27, 885-94. 11 . Hsia, H.C. et al. (2017) J Biol Chem 292, 5405-5417. |
Western blot analysis of extracts or media collected from THP-1 cells, differentiated with TPA (12-0-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, overnight), and subsequently treated with (+) or without (-) Lipopolysaccharides (LPS) #14011 (1 μg/ml, 15 min), using Phosho-Stat3 (Ser754) Antibody (upper) or Stat3 (D3Z2G) Rabbit mAb #12640 (lower). | |
Immunoprecipitation of Phospho-Stat3 (Ser754) from extracts of THP-1 cells, differentiated with TPA (12-0-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, overnight) followed by treatment with Lipopolysaccharides (LPS) #14011 (1 μg/ml, 15 min). Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is Phospho-Stat3 (Ser754) Antibody. Western blot was performed using Phospho-Stat3 (Ser754). |
