| 货号 | 67314S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Cleaved Caspase-1 (Asp296) Antibody recognizes endogenous levels of Caspase-1 protein only when cleaved at Asp296. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε and ζ (6). Most caspase-1 isoforms (α, β, γ and δ) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7). |
| 存放说明 | -20C |
| 计算分子量 | 22 |
| 参考文献 | 1 . Thornberry, N.A. et al. (1992) Nature 356, 768-74. 2 . Martinon, F. and Tschopp, J. (2004) Cell 117, 561-74. 3 . Miura, M. et al. (1993) Cell 75, 653-60. 4 . Kuida, K. et al. (1995) Science 267, 2000-3. 5 . Li, P. et al. (1995) Cell 80, 401-11. 6 . Feng, Q. et al. (2004) Genomics 84, 587-91. 7 . Martinon, F. et al. (2002) Mol Cell 10, 417-26. |
Western blot analysis of cell extracts and media from mouse bone marrow derived macrophages (mBMDM), untreated (-) or treated with Lipopolysaccharides (LPS) #14011 (50 ng/ml, 4 hr) followed by nigericin (15 μM, 45 min) (+), using Cleaved Caspase-1 (Asp296) Antibody. |
