| 货号 | 86668S |
| 反应种属 | H M |
| 来源宿主 | Rabbit |
| 应用 | W IP |
| 目标/特异性 | MFF Antibody recognizes endogenous levels of total MFF protein. Based upon sequence alignment, this antibody is predicted to react with isoforms 1-5 of human MFF protein. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mitochondrial fission factor (MFF) is a tail-anchored protein that resides within the outer mitochondrial membrane and is part of the mitochondrial fission complex. MFF participates in mitochondrial fission by serving as one of multiple receptors for the GTPase dynamin-related protein 1 (Drp1) (1-4). Research studies have also shown that MFF is a peroxisomal membrane protein and participates in peroxisome fission by serving as a receptor for another GTPase, dynamin-like protein 1 (5,6). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 25, 27, 30, 35 |
| 参考文献 | 1 . Liu, R. and Chan, D.C. (2015) Mol Biol Cell 26, 4466-77. 2 . Shen, Q. et al. (2014) Mol Biol Cell 25, 145-59. 3 . Losón, O.C. et al. (2013) Mol Biol Cell 24, 659-67. 4 . Otera, H. et al. (2010) J Cell Biol 191, 1141-58. 5 . Itoyama, A. et al. (2013) Biol Open 2, 998-1006. 6 . Gandre-Babbe, S. and van der Bliek, A.M. (2008) Mol Biol Cell 19, 2402-12. 7 . Toyama, E.Q. et al. (2016) Science 351, 275-81. 8 . Ducommun, S. et al. (2015) Cell Signal 27, 978-88. |
Western blot analysis of extracts from various cell lines using MFF Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human MFF (+), using MFF Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). | |
Immunoprecipitation of MFF from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is MFF Antibody. Western blot analysis was performed using MFF Antibody. |
