| 货号 | 41255S |
| 反应种属 | H |
| 来源宿主 | Rabbit |
| 应用 | W IP |
| 目标/特异性 | SLK antibody recognizes endogenous levels of total SLK protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SLK (Ste20-like Kinase) is a member of the germinal center kinase (GCK) family of proteins. SLK has a kinase domain located at the N terminus (1). The autophosphorylation of SLK at Thr183 and Ser189 is required for the upregulation of SLK kinase activity (1, 2). The protein also has a caspase cleavage site DXXD and a SH3 binding site PXXP located in the middle part of its sequence, and a regulatory C terminal coiled-coil domain for homodimerization and adaptor binding (1-4). SLK plays important roles in development, tissue regeneration and cancer cell migration by regulating several signaling pathways (5-7). SLK phosphorylates and activates ASK1 to induce downstream p38 phosphorylation and apoptosis (8,9). During cell cycle, SLK phosphorylates Polo-like kinase (PLK) at Thr210 to promote G2/M transition (10,11). SLK also promotes cell division by direct phosphorylation of ERMs and dynactin to activate microtubule reorganization and spindle orientation (12, 13). During focal adhesion and cell migration process, SLK is activated and colocalized to the focal adhesion complex where it promotes complex turnover by phosphorylating paxillin at Ser250 (14, 15). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 220 |
| 参考文献 | 1 . Al-Zahrani, K.N. et al. (2013) Cell Adh Migr 7, 1-10. 2 . Luhovy, A.Y. et al. (2012) J Biol Chem 287, 5446-58. 3 . Delarosa, S. et al. (2011) Am J Physiol Renal Physiol 301, F554-64. 4 . Baron, K.D. et al. (2015) Biochim Biophys Acta 1853, 1683-92. 5 . Al-Zahrani, K.N. et al. (2014) Dev Dyn 243, 640-51. 6 . Storbeck, C.J. et al. (2013) Skelet Muscle 3, 16. 7 . Roovers, K. et al. (2009) Oncogene 28, 2839-48. 8 . Hao, W. et al. (2006) J Biol Chem 281, 3075-84. 9 . Sabourin, L.A. et al. (2000) Mol Cell Biol 20, 684-96. 10 . Ellinger-Ziegelbauer, H. et al. (2000) Genes Cells 5, 491-8. 11 . Johnson, T.M. et al. (2008) Biochemistry 47, 3688-96. 12 . Zhapparova, O.N. et al. (2013) Mol Biol Cell 24, 3205-14. 13 . Machicoane, M. et al. (2014) J Cell Biol 205, 791-9. 14 . Wagner, S. et al. (2008) PLoS One 3, e1868. 15 . Quizi, J.L. et al. (2013) Oncogene 32, 4656-63. |
Western blot analysis of extracts from various cell lines using SLK Antibody. | |
Immunoprecipitation of SLK protein from HCC1419 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SLK Antibody. Western blot analysis was performed using SLK Antibody. |
