| 货号 | 34511S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | WB,IP |
| 目标/特异性 | MLANA/MART-1 Antibody recognizes endogenous levels of total MLANA/MART-1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | MLANA, also known as MART-1, is a member of a melanocyte lineage-specific family of proteins. It is expressed in melanocytes, retinal pigment epithelium, and melanoma cells. Its function is not entirely understood, but it is believed to be involved in the stability of GPR143, as well as the stability, trafficking, and processing of PMEL; both proteins are involved in the formation of stage II melanosomes (1). In melanosomes, MLANA is specifically located in the trans-Golgi network, however conformational changes to the protein or a sub-population of the protein causes it to localize back to the ER and small endosomal vesicles (2). In the context of melanoma cells, the conformational change is thought to be caused by aberrant exposure of epitopes, which are recognized by cytolytic T-lymphocytes (3). MLANA may be useful as a marker of metastatic melanoma (4). MHC-II restricted phospho-MLANA peptides, which are recognized by CD4 cells, are being investigated as potential candidates for cancer immunotherapy (5). |
| 存放说明 | -20C |
| 计算分子量 | 19 |
| 参考文献 | 1 . Hoashi, T. et al. (2005) J Biol Chem 280, 14006-16. 2 . De Mazière, A.M. et al. (2002) Traffic 3, 678-93. 3 . Rimoldi, D. et al. (2001) J Biol Chem 276, 43189-96. 4 . Wandler, A. et al. (2016) J Cutan Pathol 43, 956-962. 5 . Depontieu, F.R. et al. (2009) Proc Natl Acad Sci U S A 106, 12073-8. |
Western blot of extracts from SK-MEL-28 and OVCAR3 cells using MLANA/MART-1 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, expression in OVCAR3 is low/negative. |
