| 货号 | 60940S |
| 反应种属 | Human, Monkey |
| 来源宿主 | Rabbit |
| 应用 | WB,IP |
| 目标/特异性 | Astrin/SPAG5 Antibody recognizes endogenous levels of total Astrin/SPAG5 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Astrin/SPAG5 was identified as a microtubule-associated protein in a mitotic extract (1). It is essential for cells to assemble biploar spindle structures and progress through mitosis (1, 2). Astrin/SPAG5 was also identified to be a component associated with outer dense fibers in the sperm tail (3). In addition, this protein negatively regulates mTORC1 activity during the cell stress response (4). Under stress conditions, Astrin/SPAG5 interacts with the mTORC1 component raptor and recruits raptor to stress granules, thereby suppressing mTORC1 formation (4). The inhibition of mTORC1 prevents its hyperactivation and thus keeps cells from undergoing apoptosis during stresses (4). Furthermore, Astrin/SPAG5 has been implicated to be a prognostic marker in breast cancer (5). |
| 存放说明 | -20C |
| 计算分子量 | 135, 150 |
| 参考文献 | 1 . Mack, G.J. and Compton, D.A. (2001) Proc Natl Acad Sci U S A 98, 14434-9. 2 . Gruber, J. et al. (2002) J Cell Sci 115, 4053-9. 3 . Xue, J. et al. (2002) Mol Cell Biol 22, 1993-7. 4 . Thedieck, K. et al. (2013) Cell 154, 859-74. 5 . Abdel-Fatah, T.M. et al. (2016) Lancet Oncol 17, 1004-18. |
Western blot analysis of extracts from various cell lines using Astrin/SPAG5 Antibody. |
