| 货号 | 84336S |
| 反应种属 | Human, Mouse, Rat |
| 来源宿主 | Rabbit |
| 应用 | WB,IP |
| 目标/特异性 | COL1A1 Antibody recognizes endogenous levels of total COL1A1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Type 1 collagen is the most abundant collagen in many human tissues, including bone, skin, and tendons. It is a trimeric complex comprised of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen) (1-3). The expression levels of COL1A1 are regulated by multiple mechanisms, including mRNA stability, translation, and posttranslational modification (3-5). Overexpression of COL1A1 has been positively associated with tissue fibrosis disorders, including systemic sclerosis (6), while loss-of-function mutations in the COL1A1 gene are a major causative factor for osteogenesis imperfecta (brittle bone disease) (7). Notably, COL1A1 expression levels have also been associated with tumor development in gastric, lung, thyroid, and breast cancers. Research studies suggest that upregulation of COL1A1 can generate a modified extracellular matrix environment that promotes cancer cell survival, proliferation, metastasis, and invasion (8-11). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
| 参考文献 | 1 . Prockop, D.J. and Kivirikko, K.I. (1995) Annu Rev Biochem 64, 403-34. 2 . Chang, S.W. et al. (2012) Biophys J 102, 640-8. 3 . Zhang, Y. and Stefanovic, B. (2016) Int J Mol Sci 17, 419. 4 . Parsons, C.J. et al. (2011) J Biol Chem 286, 8609-19. 5 . Cai, L. et al. (2010) J Mol Biol 395, 309-26. 6 . Jimenez, S.A. and Saitta, B. (1999) Springer Semin Immunopathol 21, 397-414. 7 . Forlino, A. et al. (2011) Nat Rev Endocrinol 7, 540-57. 8 . Li, J. et al. (2016) World J Surg Oncol 14, 297. 9 . Oleksiewicz, U. et al. (2017) J Cancer Res Clin Oncol. 10 . Barcus, C.E. et al. (2017) Breast Cancer Res 19, 9. 11 . Jolly, L.A. et al. (2016) Cancer Res 76, 1804-13. |
Western blot analysis of extracts from various cell lines using COL1A1 Antibody. | |
Immunoprecipitation of COL1A1 protein from U118 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is COL1A1 Antibody. Western blot analysis was performed using COL1A1 Antibody. |
