| 货号 | 2309S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | γ-Catenin Antibody detects endogenous levels ot total γ-catenin protein. The antibody does not cross-react with β-catenin. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Also known as plakoglobin, γ-catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the Drosophila protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9). |
| 存放说明 | -20C |
| 计算分子量 | 83 |
| 参考文献 | 1 . Zhurinsky, J. et al. (2000) J Cell Sci 113 ( Pt 18), 3127-39. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Zhurinsky, J. et al. (2000) Mol Cell Biol 20, 4238-52. 5 . Charpentier, E. et al. (2000) J Cell Biol 149, 503-20. 6 . Kolligs, F.T. et al. (2000) Genes Dev 14, 1319-31. 7 . Miravet, S. et al. (2003) Mol Cell Biol 23, 7391-402. 8 . Hu, P. et al. (2006) J Biol Chem 281, 12786-91. 9 . Yin, T. et al. (2005) J Biol Chem 280, 40355-63. |
Western blot analysis of extracts from 293, MCF-7 and C2C12 cells using γ-Catenin Antibody.使用γ-Catenin抗体对293， MCF-7和C2C12细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcioma using gamma-Catenin Antibody.使用gamma-Catenin抗体对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using gamma-Catenin Antibody.使用gamma-Catenin抗体对石蜡包埋的人肺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcioma using gamma-Catenin Antibody.使用gamma-Catenin抗体对石蜡包埋的人结肠癌组织进行免疫组化分析。 | |
Confocal immunofluorescent analysis of HT-29 cells labeled with gamma-Catenin Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).使用gamma-Catenin抗体（绿色）对HT-29细胞进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5™（DNA荧光染料）。 |
