| 货号 | 2719S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | βIG-H3 Antibody detects endogenous levels of total βIG-H3 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | βIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683-amino acid secretory protein induced by TGF-β that plays a role in cell adhesion, differentiation, and apoptosis (1-4). βIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM βIG-H3 is found in a wide variety of tissues (8-12). Mutations in the βIG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
| 参考文献 | 1 . Skonier, J. et al. (1992) DNA Cell Biol 11, 511-22. 2 . Skonier, J. et al. (1994) DNA Cell Biol 13, 571-84. 3 . Hashimoto, K. et al. (1997) Biochim Biophys Acta 1355, 303-14. 4 . Kim, J.E. et al. (2003) Oncogene 22, 2045-53. 5 . Kim, J.E. et al. (2002) Invest Ophthalmol Vis Sci 43, 656-61. 6 . Billings, P.C. et al. (2002) J Biol Chem 277, 28003-9. 7 . Hanssen, E. et al. (2003) J Biol Chem 278, 24334-41. 8 . Gibson, M.A. et al. (1997) J Histochem Cytochem 45, 1683-96. 9 . Billings, P.C. et al. (2000) Am J Respir Cell Mol Biol 22, 352-9. 10 . Gilbert, R.E. et al. (1998) Kidney Int 54, 1052-62. 11 . Rawe, I.M. et al. (1997) Invest Ophthalmol Vis Sci 38, 893-900. 12 . LeBaron, R.G. et al. (1995) J Invest Dermatol 104, 844-9. 13 . Munier, F.L. et al. (1997) Nat Genet 15, 247-51. |
Confocal immunofluorescent analysis of A549 cells, untreated (left) or TGF-β-treated (right), using βIG-H3 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).未处理（左）或 TGF-β处理（右）的A549细胞使用βIG-H3 抗体（绿色）进行激光共聚焦免疫荧光分析。肌动蛋白丝使用 Alexa Fluor® 555 鬼笔环肽（红色）进行标记。蓝色假色=DRAQ5™（DNA荧光染料）。 | |
Western blot analysis of extracts from A549 and OVCAR8 cells, untreated or treated with TGF-β overnight, using βIG-H3 Antibody.未处理或使用TGF-β 处理过夜的 A549 和OVCAR8细胞提取物，使用βIG-H3 抗体进行western blot分析。 |
