β-Catenin Antibody (Carboxy-terminal Antigen)【科瑞奥博】

产品中心

当前位置：首页>产品中心

β-Catenin Antibody (Carboxy-terminal Antigen)

货号： 9587S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	9587S
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Rabbit
应用	W/IP/IHC-P/IHC-F/ChIP
目标/特异性	Beta-Catenin Antibody detects endogenous levels of total β-catenin protein.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:1600) IHC-F (1:400) ChIP (1:25)

性能
供应商	CST
灵敏度	Endogenous
背景	β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
存放说明	-20C
计算分子量	92
参考文献	1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5 . Liu, C. et al. (2002) Cell 108, 837-47. 6 . Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7 . Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8 . Morin, P.J. et al. (1997) Science 275, 1787-90.

参考图片

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen) in the presence of control peptide (left) or beta-Catenin blocking peptide #1002 (right).

在对照多肽（左）或beta-Catenin封闭多肽#1002存在的条件下，使用 β-Catenin抗体（羧基末端抗原）对石蜡包埋的人乳腺癌组织进行免疫组化分析。

Western blot analysis of total cell extracts from 293 and NIH/3T3 cells using β-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体（羧基末端抗原）对293和NIH/3T3细胞提取物进行western blot分析。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin Antibody (Carboxy-terminal Antigen).

beta-Catenin对石蜡包埋的人结肠癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体（羧基末端抗原）对石蜡包埋的人肺癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen).

使用beta-Catenin对石蜡包埋的人透明细胞癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma using β-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体（羧基末端抗原）对石蜡包埋的Apc（Min/+）小鼠肠腺瘤组织进行免疫组化分析。

Immunohistochemical analysis of frozen HCC827 xenograft using beta-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体（羧基末端抗原）对冰冻的HCC827爪蟾进行免疫组化分析。

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells and either 20 μl of β-Catenin Antibody (Carboxy-terminal Antigen) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.